《植物生理学报》 2010, 46(4): 365-369

不同氮素水平下小黑麦旗叶蛋白质差异表达

李晶, 吉彪, 商文楠, 陈龙涛, 魏玲, 魏湜*

东北农业大学农学院, 哈尔滨150030

通信作者：魏湜；E-mail: weishi5608@163.com；Tel: 0451-55191599

摘　要：

在不同氮素水平下, 研究小黑麦叶片蛋白质差异表达, 进行 MALDI-TOF-MS 和生物信息学分析。找到 7 个差异表 达蛋白, 即与蛋白质合成和信号传导相关的蛋白、与清除自由基有关的酶、与叶绿体的发育和代谢有关蛋白、与植物碳代 谢有关的酶、与氮素同化有关的酶。氮素对植物生长发育、物质代谢和信号传导影响全面, 从蛋白点的鉴定分析来看, 功 能蛋白是其影响的重点。

关键词：小黑麦; 差异表达; 双向电泳; 氮素

收稿：2010-01-25   修定：2010-02-08

资助：黑龙江省农业委员会项目(GC05B708)

Protein Differential Expression under Different Nitrogen Application Treatment in Triticale

LI Jing, JI Biao, SHANG Wen-Nan, CHEN Long-Tao, WEI Ling, WEI Shi*

College of Agriculture, Northeast Agricultural University, Harbin 150030, China

Corresponding author: WEI Shi; E-mail: weishi5608@163.com; Tel: 0451-55191599

Abstract:

Proteins differential expression under different nitrogen levels were analyzed in triticale. Seven differentially expressed proteins were found by MALDI-TOF-MS and bioinformatics analysis, including proteins related with protein synthesis and signal transduction, enzyme related with elimination of the free radical, proteins related with chloroplast growth and metabolism, enzyme related with carbon metabolism, and enzyme related with nitrogen assimilation. Nitrogen had overall effects on growth, substance metabolism and signal conduction of plant. Identification and analysis on protein spots showed that the emphasis of its influence was functional proteins.

Key words: triticale; differential expression; dimensional electrophoresis; nitrogen